FastQCFastQC Report
水 19 2 2020
2-2_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2-2_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16415732
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC1944571.1845770873939705No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1685081.0265031129894178No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG1273770.7759446852568013No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT1075180.6549692697224833No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG1070970.6524046567037035No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT947800.5773729736815879No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG840090.5117590857355614No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG760110.46303753009612975No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT734050.44716251459270895No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA678250.41317073158845424No Hit
GTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGC678000.4130184386538474No Hit
GTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAG659570.4017914035146285No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG562760.3428174875174619No Hit
TGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGA549910.3349896306786685No Hit
GGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGA526950.32100304756437303No Hit
GCTTTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG526000.32042433441286683No Hit
GGTTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG503970.30700428101530897No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA489950.29846369324255534No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC488700.297702228569521No Hit
GTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATT423760.25814261587603893No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA419490.25554145255295346No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA409730.24959593638590105No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT408480.24883447171286668No Hit
GGGGATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG386240.23528649225023895No Hit
GGTAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT374990.22843331019292956No Hit
TGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTT360030.21932010098605412No Hit
GTTGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT358710.2185159942913298No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG352050.21445891051340263No Hit
GGTCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG335990.2046756123942569No Hit
GGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAA328810.20030175931234745No Hit
GGTGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT319220.1944598023408277No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT317050.19313789966844No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA312600.1904270854324376No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA312600.1904270854324376No Hit
GGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGG311000.1894524106509536No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA310870.18937321832495804No Hit
GGGTGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT299520.18245911909380588No Hit
GTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAA278060.16938629358715163No Hit
GTGTGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT277290.16891723134856246No Hit
GTTTATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG268930.16382455561530854No Hit
GGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTG264650.16121730057483882No Hit
GTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGC255700.15576521351591266No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA249630.15206754106365772No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC244530.14896076519767745No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT231190.14083441420705453No Hit
TGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCT230500.14041408570753958No Hit
GGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAA215000.13097192376191327No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC208910.12726206787488978No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT202840.12356439542263481No Hit
GTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGC200400.1220780163808717No Hit
CTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG193210.11769807158157797No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG190540.11607158303997653No Hit
TGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGAC190070.1157852723229156No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG189240.11527965978002078No Hit
GTTCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG187820.1144146359114537No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA182720.11130786004547345No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT171830.10467397981399794No Hit
GGTTGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT171010.10417445898848739No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA168940.10291347348994244No Hit
GGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGA164340.10011128349317594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)